Human TNFRSF1B(Tumor Necrosis Factor Receptor Superfamily, Member 1B) Microsample ELISA Kit

Artikelnummer: EBT-ELK1346MS
Artikelname: Human TNFRSF1B(Tumor Necrosis Factor Receptor Superfamily, Member 1B) Microsample ELISA Kit
Artikelnummer: EBT-ELK1346MS
Hersteller Artikelnummer: ELK1346MS
Alternativnummer: EBT-ELK1346MS-96, EBT-ELK1346MS-48, EBT-ELK1346MS-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Human
Alternative Synonym: CD120B, P75, TBPII, TNF-R-II, TNF-R75, TNFBR, TNFR2, TNFR80, P75tnfr, Tumor necrosis factor receptor 2, p80 TNF-alpha receptor, Etanercept
Konzentration: 10 ng/mL
Detektionsbereich: 0.16-10 ng/mL
Sensitivitaet: 0.062 ng/mL
UniProt: P20333
Proben: Serum, plasma, tissue homogenates and other biological fluids
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Signal transduction,CD & Adhesion molecule,Apoptosis,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TNFRSF1B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TNFRSF1B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TNFRSF1B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human TNFRSF1B in the samples is then determined by comparing the OD of the samples to the standard curve
ELK1346MS