Mouse MFGE8(Milk Fat Globule EGF Factor 8) ELISA Kit

Artikelnummer: EBT-ELK1379
Artikelname: Mouse MFGE8(Milk Fat Globule EGF Factor 8) ELISA Kit
Artikelnummer: EBT-ELK1379
Hersteller Artikelnummer: ELK1379
Alternativnummer: EBT-ELK1379-96, EBT-ELK1379-48, EBT-ELK1379-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Mouse
Alternative Synonym: HMFG, MFGM, SED1, BA46, EDIL1, OAcGD3S, SPAG10, hP47, Medin, Lactadherin, Sperm Associated Antigen 10, Sperm Surface Protein hP47, Milk fat globule-EGF factor 8
Konzentration: 1000 pg/mL
Detektionsbereich: 15.63-1000 pg/mL
Sensitivitaet: 6 pg/mL
UniProt: P21956
Proben: Serum, plasma, tissue homogenates and other biological fluids
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Tumor immunity,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MFGE8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MFGE8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MFGE8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Mouse MFGE8 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK1379