Human C4BPa(C4 Binding Protein Alpha) Microsample ELISA Kit

Artikelnummer: EBT-ELK1594MS
Artikelname: Human C4BPa(C4 Binding Protein Alpha) Microsample ELISA Kit
Artikelnummer: EBT-ELK1594MS
Hersteller Artikelnummer: ELK1594MS
Alternativnummer: EBT-ELK1594MS-96, EBT-ELK1594MS-48, EBT-ELK1594MS-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Human
Alternative Synonym: C4BP-a, PRP, C4BP, Complement Component 4 Binding Protein,Alpha, Proline-rich protein
Konzentration: 10 ng/mL
Detektionsbereich: 0.16-10 ng/mL
Sensitivitaet: 0.068 ng/mL
UniProt: P04003
Proben: Serum, plasma and other biological fluids
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Immune molecule,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C4BPa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C4BPa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C4BPa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human C4BPa in the samples is then determined by comparing the OD of the samples to the standard curve
ELK1594MS