Chicken IL6(Interleukin 6) ELISA Kit

Artikelnummer: EBT-ELK1607
Artikelname: Chicken IL6(Interleukin 6) ELISA Kit
Artikelnummer: EBT-ELK1607
Hersteller Artikelnummer: ELK1607
Alternativnummer: EBT-ELK1607-96, EBT-ELK1607-48, EBT-ELK1607-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Gallus
Alternative Synonym: IL-6,MGI2-A, MGI2A, HGF, BSF2, HSF, IFNB2, B-Cell Stimulatory Factor-2, Hybridoma/Plasmacytoma Growth Factor, Hepatocyte Stimulating Factor, Cytotoxic T-Cell Differentiation Factor
Konzentration: 1000 pg/mL
Detektionsbereich: 15.63-1000 pg/mL
Sensitivitaet: 5.5 pg/mL
UniProt: Q90YI0
Proben: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Cytokine,Tumor immunity,Infection immunity,Cardiovascular biology,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken IL6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken IL6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken IL6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Chicken IL6 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK1607