Rat PPAR-gamma(Peroxisome Proliferator Activated Receptor Gamma) ELISA Kit

Produktübersicht

• Artikelname: Rat PPAR-gamma(Peroxisome Proliferator Activated Receptor Gamma) ELISA Kit
• Artikelnummer: EBT-ELK1632
• Hersteller Artikelnummer: ELK1632
• Alternativnummer: EBT-ELK1632-96, EBT-ELK1632-48, EBT-ELK1632-96X5
• Hersteller: ELK Biotechnology
• Kategorie: Kits/Assays
• Spezies Reaktivität: Rat
• Alternative Synonym: PPAR-G, PPARG1, PPARG2, NR1C3, Glitazone Receptor, Nuclear Receptor Subfamily 1 Group C Member 3, PPARg, Peroxisome Proliferator Activated Receptor Gamma

Produkteigenschaften

• Konzentration: 10 ng/mL
• Detektionsbereich: 0.16-10 ng/mL
• Sensitivitaet: 0.059 ng/mL
• UniProt: O88275
• Proben: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Anwendungsbeschreibung

• Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Metabolic pathway,Endocrinology,Cardiovascular biology,Developmental science,Bone metabolism,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PPAR-gamma. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PPAR-gamma. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PPAR-gamma, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat PPAR-gamma in the samples is then determined by comparing the OD of the samples to the standard curve

Bilder

ELK1632