Mouse GZMB(Granzyme B) Microsample ELISA Kit

Artikelnummer: EBT-ELK1660MS
Artikelname: Mouse GZMB(Granzyme B) Microsample ELISA Kit
Artikelnummer: EBT-ELK1660MS
Hersteller Artikelnummer: ELK1660MS
Alternativnummer: EBT-ELK1660MS-96, EBT-ELK1660MS-48, EBT-ELK1660MS-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Mouse
Alternative Synonym: GZM-B, HLP, CTLA1, CCPI, CGL1, CSP-B, CSPB, CTSGL1, SECT, Granzyme 2, Cytotoxic T-Lymphocyte-Associated Serine Esterase 1, Fragmentin 2, Cytotoxic Serine Protease B
Konzentration: 2000 ng/mL
Detektionsbereich: 31.25-2000 ng/mL
Sensitivitaet: 13.81 ng/mL
UniProt: P04187
Proben: Serum, plasma, tissue homogenates and other biological fluids
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Apoptosis,Tumor immunity,Infection immunity,Immune molecule,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GZMB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GZMB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GZMB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Mouse GZMB in the samples is then determined by comparing the OD of the samples to the standard curve
ELK1660MS