Human CYFRA21-1(Cytokeratin Fragment Antigen 21-1) ELISA Kit
Artikelnummer:
EBT-ELK1967
Hersteller Artikelnummer:
ELK1967
Alternativnummer:
EBT-ELK1967-96, EBT-ELK1967-48, EBT-ELK1967-96X5
Hersteller:
ELK Biotechnology
Kategorie:
Kits/Assays
Spezies Reaktivität:
Human
Alternative Synonym:
CYFRA21-1
Konzentration:
20 ng/mL
Detektionsbereich:
0.32-20 ng/mL
Sensitivitaet:
0.117 ng/mL
Proben:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Anwendungsbeschreibung:
Assay Type: Sandwich. Assay length: 3.5h. Research Area: Tumor immunity,Dermatology,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CYFRA21-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CYFRA21-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CYFRA21-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human CYFRA21-1 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK1967
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