Human CHRNb2(Cholinergic Receptor, Nicotinic, Beta 2) ELISA Kit

Artikelnummer: EBT-ELK2057
Artikelname: Human CHRNb2(Cholinergic Receptor, Nicotinic, Beta 2) ELISA Kit
Artikelnummer: EBT-ELK2057
Hersteller Artikelnummer: ELK2057
Alternativnummer: EBT-ELK2057-96, EBT-ELK2057-48, EBT-ELK2057-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Human
Alternative Synonym: CHR-NB2, CHRN-B2, EFNL3, NAChRB2, N-AChR-B2, N-AChRB2, Neuronal Acetylcholine Receptor Beta 2
Konzentration: 10 ng/mL
Detektionsbereich: 0.16-10 ng/mL
Sensitivitaet: 0.056 ng/mL
UniProt: P17787
Proben: Tissue homogenates and other biological fluids.
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Signal transduction,Neuro science,Developmental science,Bone metabolism,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CHRNb2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CHRNb2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CHRNb2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human CHRNb2 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK2057