Human FcgRI(Receptor I For The Fc Region Of Immunoglobulin G) Microsample ELISA Kit

Artikelnummer: EBT-ELK2070MS
Artikelname: Human FcgRI(Receptor I For The Fc Region Of Immunoglobulin G) Microsample ELISA Kit
Artikelnummer: EBT-ELK2070MS
Hersteller Artikelnummer: ELK2070MS
Alternativnummer: EBT-ELK2070MS-96, EBT-ELK2070MS-48, EBT-ELK2070MS-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Human
Alternative Synonym: CD64, FCGR1, FCGR1A, FCGR1B, FCGR1C, FcgRIA, CD64A, FcgRIB, CD64B, FcgRIC, CD64C, High affinity immunoglobulin gamma Fc receptor I
Konzentration: 5000 pg/mL
Detektionsbereich: 78.13-5000 pg/mL
Sensitivitaet: 32 pg/mL
UniProt: P12314
Proben: serum, plasma, tissue homogenates and other biological fluids
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: CD & Adhesion molecule,Infection immunity,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FcgRI. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FcgRI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FcgRI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human FcgRI in the samples is then determined by comparing the OD of the samples to the standard curve
ELK2070MS