Human CCR7(Chemokine C-C-Motif Receptor 7) ELISA Kit

Artikelnummer: EBT-ELK2135
Artikelname: Human CCR7(Chemokine C-C-Motif Receptor 7) ELISA Kit
Artikelnummer: EBT-ELK2135
Hersteller Artikelnummer: ELK2135
Alternativnummer: EBT-ELK2135-96, EBT-ELK2135-48, EBT-ELK2135-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Human
Alternative Synonym: CD197, CCR7, BLR2, CDw197, CMKBR7, EBI1, Epstein-Barr Virus Induced Protein 1, EBV-induced G-protein coupled receptor 1, MIP-3 beta receptor
Konzentration: 10 ng/mL
Detektionsbereich: 0.16-10 ng/mL
Sensitivitaet: 0.06 ng/mL
UniProt: P32248
Proben: Tissue homogenates and other biological fluids.
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Signal transduction,CD & Adhesion molecule,Tumor immunity,Infection immunity,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CCR7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CCR7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CCR7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human CCR7 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK2135