Human HMGCS(Hydroxymethylglutaryl Coenzyme A Synthase) ELISA Kit

Produktübersicht

• Artikelname: Human HMGCS(Hydroxymethylglutaryl Coenzyme A Synthase) ELISA Kit
• Artikelnummer: EBT-ELK2172
• Hersteller Artikelnummer: ELK2172
• Alternativnummer: EBT-ELK2172-96, EBT-ELK2172-48, EBT-ELK2172-96X5
• Hersteller: ELK Biotechnology
• Kategorie: Kits/Assays
• Spezies Reaktivität: Human
• Alternative Synonym: HMGCS1, 3-Hydroxy-3-Methylglutaryl-Coenzyme A Synthase 1,Soluble, HMG-CoA Synthase

Produkteigenschaften

• Konzentration: 10 ng/mL
• Detektionsbereich: 0.16-10 ng/mL
• Sensitivitaet: 0.053 ng/mL
• UniProt: Q01581
• Proben: Tissue homogenates and other biological fluids.

Anwendungsbeschreibung

• Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Enzyme & Kinase,Metabolic pathway,Endocrinology,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HMGCS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HMGCS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HMGCS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human HMGCS in the samples is then determined by comparing the OD of the samples to the standard curve

Bilder

ELK2172