Human MIP3b(Macrophage Inflammatory Protein 3 Beta) Microsample ELISA Kit

Artikelnummer: EBT-ELK2181MS
Artikelname: Human MIP3b(Macrophage Inflammatory Protein 3 Beta) Microsample ELISA Kit
Artikelnummer: EBT-ELK2181MS
Hersteller Artikelnummer: ELK2181MS
Alternativnummer: EBT-ELK2181MS-96, EBT-ELK2181MS-48, EBT-ELK2181MS-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Human
Alternative Synonym: CCL19, CKb11, ELC, MIP3-B, SCYA19, Chemokine C-C-Motif Ligand 19, Beta Chemokine Exodus-3, CK Beta-11, EBI1-Ligand Chemokine, Small Inducible Cytokine Subfamily A19
Konzentration: 1000 pg/mL
Detektionsbereich: 15.63-1000 pg/mL
Sensitivitaet: 6.3 pg/mL
UniProt: Q99731
Proben: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Cytokine,Infection immunity,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MIP3b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MIP3b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MIP3b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human MIP3b in the samples is then determined by comparing the OD of the samples to the standard curve
ELK2181MS