Mouse PTGS2/COX-2(Prostaglandin Endoperoxide Synthase 2) ELISA Kit

Produktübersicht

• Artikelname: Mouse PTGS2/COX-2(Prostaglandin Endoperoxide Synthase 2) ELISA Kit
• Artikelnummer: EBT-ELK2231
• Hersteller Artikelnummer: ELK2231
• Alternativnummer: EBT-ELK2231-96,EBT-ELK2231-48,EBT-ELK2231-96X5
• Hersteller: ELK Biotechnology
• Kategorie: Kits/Assays
• Spezies Reaktivität: Mouse
• Alternative Synonym: PGG/HS, PGHS2, PHS2, HCox2, COX2, Cyclooxygenase 2, Prostaglandin G/H Synthase And Cyclooxygenase, Prostaglandin H2 synthase 2, PTGS2, Prostaglandin Endoperoxide Synthase 2

Produkteigenschaften

• Konzentration: 5000 pg/mL
• Detektionsbereich: 78.13-5000 pg/mL
• Sensitivitaet: 35 pg/mL
• UniProt: Q05769
• Proben: serum, plasma and other biological fluids

Anwendungsbeschreibung

• Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Enzyme & Kinase,Tumor immunity,Infection immunity,Reproductive science,Pulmonology,Gastroenterology,Hormone metabolism,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PTGS2/COX-2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PTGS2/COX-2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PTGS2/COX-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Mouse PTGS2/COX-2 in the samples is then determined by comparing the OD of the samples to the standard curve