Human EMILIN1(Elastin Microfibril Interface Located Protein 1) Microsample ELISA Kit

Artikelnummer: EBT-ELK2362MS
Artikelname: Human EMILIN1(Elastin Microfibril Interface Located Protein 1) Microsample ELISA Kit
Artikelnummer: EBT-ELK2362MS
Hersteller Artikelnummer: ELK2362MS
Alternativnummer: EBT-ELK2362MS-96, EBT-ELK2362MS-48, EBT-ELK2362MS-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Human
Alternative Synonym: EMI, gp115, Elastin Microfibril Interfacer 1
Konzentration: 100 ng/mL
Detektionsbereich: 1.57-100 ng/mL
Sensitivitaet: 0.59 ng/mL
UniProt: Q9Y6C2
Proben: serum, plasma, tissue homogenates and other biological fluids
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Metabolic pathway,Cardiovascular biology,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EMILIN1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EMILIN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EMILIN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human EMILIN1 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK2362MS