Human TRH(Thyrotropin Releasing Hormone) ELISA Kit

Produktübersicht

• Artikelname: Human TRH(Thyrotropin Releasing Hormone) ELISA Kit
• Artikelnummer: EBT-ELK2641
• Hersteller Artikelnummer: ELK2641
• Alternativnummer: EBT-ELK2641-96, EBT-ELK2641-48, EBT-ELK2641-96X5
• Hersteller: ELK Biotechnology
• Kategorie: Kits/Assays
• Spezies Reaktivität: Human
• Alternative Synonym: TRF, Thyrotropin-Releasing Factor, Thyroliberin, Protirelin, Pro-tirelin, Prothyroliberin, TSH-releasing factor

Produkteigenschaften

• Konzentration: 500 pg/mL
• Detektionsbereich: 7.82-500 pg/mL
• Sensitivitaet: 2.7 pg/mL
• UniProt: P20396
• Proben: serum, plasma and other biological fluids

Anwendungsbeschreibung

• Anwendungsbeschreibung: Assay Type: Competitive Inhibition. Assay length: 2h. Research Area: Endocrinology,Neuro science,Hepatology,Hormone metabolism,. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human TRH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human TRH in the samples is then determined by comparing the OD of the samples to the standard curve

Bilder

ELK2641