Mouse ADCYAP1(Adenylate Cyclase Activating Polypeptide 1, Pituitary) ELISA Kit

Produktübersicht

• Artikelname: Mouse ADCYAP1(Adenylate Cyclase Activating Polypeptide 1, Pituitary) ELISA Kit
• Artikelnummer: EBT-ELK2658
• Hersteller Artikelnummer: ELK2658
• Alternativnummer: EBT-ELK2658-96, EBT-ELK2658-48, EBT-ELK2658-96X5
• Hersteller: ELK Biotechnology
• Kategorie: Kits/Assays
• Spezies Reaktivität: Mouse
• Alternative Synonym: PACAP, PRP-48, Pituitary Adenylate Cyclase Activating Peptide

Produkteigenschaften

• Konzentration: 500 pg/mL
• Detektionsbereich: 7.82-500 pg/mL
• Sensitivitaet: 3.1 pg/mL
• Proben: Serum, plasma, tissue homogenates and other biological fluids

Anwendungsbeschreibung

• Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Endocrinology,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ADCYAP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ADCYAP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ADCYAP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Mouse ADCYAP1 in the samples is then determined by comparing the OD of the samples to the standard curve

Bilder

ELK2658