Mouse EGFR2(Epidermal Growth Factor Receptor 2) ELISA Kit

Artikelnummer: EBT-ELK2659
Artikelname: Mouse EGFR2(Epidermal Growth Factor Receptor 2) ELISA Kit
Artikelnummer: EBT-ELK2659
Hersteller Artikelnummer: ELK2659
Alternativnummer: EBT-ELK2659-96, EBT-ELK2659-48, EBT-ELK2659-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Mouse
Alternative Synonym: CD340, ErbB2, HER-2/neu, HER2, NEU, MLN 19, NGL, TKR1, p185, V-Erb-B2 Erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/Glioblastoma Derived Oncogene Homolog
Konzentration: 20 ng/mL
Detektionsbereich: 0.32-20 ng/mL
Sensitivitaet: 0.121 ng/mL
Proben: serum, plasma, tissue homogenates and other biological fluids
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Signal transduction,Tumor immunity,Reproductive science,Hepatology,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse EGFR2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse EGFR2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse EGFR2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Mouse EGFR2 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK2659