Human LXRa(Liver X Receptor Alpha) ELISA Kit

Artikelnummer: EBT-ELK2764
Artikelname: Human LXRa(Liver X Receptor Alpha) ELISA Kit
Artikelnummer: EBT-ELK2764
Hersteller Artikelnummer: ELK2764
Alternativnummer: EBT-ELK2764-96, EBT-ELK2764-48, EBT-ELK2764-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Human
Alternative Synonym: NR1-H3, LXR-A, LXRA, RLD-1, Nuclear Receptor Subfamily 1,Group H,Member 3, Oxysterols receptor LXR-alpha
Konzentration: 10 ng/mL
Detektionsbereich: 0.16-10 ng/mL
Sensitivitaet: 0.055 ng/mL
UniProt: Q13133
Proben: Tissue homogenates, cell lysates and other biological fluids.
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Signal transduction,Metabolic pathway,Infection immunity,Hepatology,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LXRa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LXRa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LXRa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human LXRa in the samples is then determined by comparing the OD of the samples to the standard curve
ELK2764