Human PDGFRb(Platelet Derived Growth Factor Receptor Beta) ELISA Kit

Artikelnummer: EBT-ELK2769
Artikelname: Human PDGFRb(Platelet Derived Growth Factor Receptor Beta) ELISA Kit
Artikelnummer: EBT-ELK2769
Hersteller Artikelnummer: ELK2769
Alternativnummer: EBT-ELK2769-96, EBT-ELK2769-48, EBT-ELK2769-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Human
Alternative Synonym: CD140b, JTK12, PDGF-R-Beta, PDGFR, PDGFR1, CD140 antigen-like family member B, Platelet-derived growth factor receptor 1, Beta-type platelet-derived growth factor receptor
Konzentration: 10 ng/mL
Detektionsbereich: 0.16-10 ng/mL
Sensitivitaet: 0.052 ng/mL
UniProt: P09619
Proben: tissue homogenates, cell lysates and other biological fluids
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Signal transduction,Tumor immunity,Infection immunity,Hematology,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PDGFRb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PDGFRb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PDGFRb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human PDGFRb in the samples is then determined by comparing the OD of the samples to the standard curve
ELK2769