Rat CX43(Connexin 43) Microsample ELISA Kit

Produktübersicht

• Artikelname: Rat CX43(Connexin 43) Microsample ELISA Kit
• Artikelnummer: EBT-ELK2881MS
• Hersteller Artikelnummer: ELK2881MS
• Alternativnummer: EBT-ELK2881MS-96, EBT-ELK2881MS-48, EBT-ELK2881MS-96X5
• Hersteller: ELK Biotechnology
• Kategorie: Kits/Assays
• Spezies Reaktivität: Rat
• Alternative Synonym: ODDD, GJAL, GJA1, GJ-A1, Gap junction 43 kDa heart protein, Gap Junction Protein Alpha 1, Oculodentodigital Dysplasia, Syndactyly Type III

Produkteigenschaften

• Konzentration: 20 ng/mL
• Detektionsbereich: 0.32-20 ng/mL
• Sensitivitaet: 0.117 ng/mL
• UniProt: P08050
• Proben: tissue homogenates, cell lysates and other biological fluids

Anwendungsbeschreibung

• Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Cardiovascular biology,Neuro science,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CX43. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CX43. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CX43, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat CX43 in the samples is then determined by comparing the OD of the samples to the standard curve

Bilder

ELK2881MS