Human RANk(Receptor Activator Of Nuclear Factor Kappa B) ELISA Kit

Artikelnummer: EBT-ELK2934
Artikelname: Human RANk(Receptor Activator Of Nuclear Factor Kappa B) ELISA Kit
Artikelnummer: EBT-ELK2934
Hersteller Artikelnummer: ELK2934
Alternativnummer: EBT-ELK2934-96, EBT-ELK2934-48, EBT-ELK2934-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Human
Alternative Synonym: CD265, TNFRSF11A, TNFRSF11-A, ODFR, OFE, TRANCER, Osteoclast differentiation factor receptor, Tumor Necrosis Factor Receptor Superfamily,Member 11a,NFKB Activator
Konzentration: 10 ng/mL
Detektionsbereich: 0.16-10 ng/mL
Sensitivitaet: 0.057 ng/mL
UniProt: Q9Y6Q6
Proben: Tissue homogenates, cell lysates and other biological fluids
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Signal transduction,Metabolic pathway,Bone metabolism,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human RANk. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human RANk. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human RANk, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human RANk in the samples is then determined by comparing the OD of the samples to the standard curve
ELK2934