Human FASN(Fatty Acid Synthase) ELISA Kit

Artikelnummer: EBT-ELK3009
Artikelname: Human FASN(Fatty Acid Synthase) ELISA Kit
Artikelnummer: EBT-ELK3009
Hersteller Artikelnummer: ELK3009
Alternativnummer: EBT-ELK3009-96, EBT-ELK3009-48, EBT-ELK3009-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Human
Alternative Synonym: FAS, OA519, SDR27X1, Short Chain Dehydrogenase/Reductase Family 27X,Member 1, S-acetyltransferase, 3-oxoacyl synthase, Oleoyl hydrolase, Enoyl-acyl-carrier-protein reductase
Konzentration: 20 ng/mL
Detektionsbereich: 0.32-20 ng/mL
Sensitivitaet: 0.111 ng/mL
UniProt: P49327
Proben: serum, plasma, tissue homogenates and other biological fluids
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Enzyme & Kinase,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FASN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FASN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FASN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human FASN in the samples is then determined by comparing the OD of the samples to the standard curve
ELK3009