Human FLNb(Filamin B Beta) ELISA Kit

Artikelnummer: EBT-ELK3056
Artikelname: Human FLNb(Filamin B Beta) ELISA Kit
Artikelnummer: EBT-ELK3056
Hersteller Artikelnummer: ELK3056
Alternativnummer: EBT-ELK3056-96, EBT-ELK3056-48, EBT-ELK3056-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Human
Alternative Synonym: ABP-278, AOI, FH1, FLN1L, LRS1, SCT, TABP, TAP, Actin Binding Protein 278, Actin-binding-like protein, Thyroid autoantigen, Filamin-3, Truncated actin-binding protein
Konzentration: 1000 pg/mL
Detektionsbereich: 15.63-1000 pg/mL
Sensitivitaet: 5.2 pg/mL
UniProt: O75369
Proben: Tissue homogenates, cell lysates and other biological fluids.
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Developmental science,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FLNb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FLNb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FLNb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human FLNb in the samples is then determined by comparing the OD of the samples to the standard curve
ELK3056