Human MYH10(Myosin Heavy Chain 10, Non Muscle) Microsample ELISA Kit

Artikelnummer: EBT-ELK3060MS
Artikelname: Human MYH10(Myosin Heavy Chain 10, Non Muscle) Microsample ELISA Kit
Artikelnummer: EBT-ELK3060MS
Hersteller Artikelnummer: ELK3060MS
Alternativnummer: EBT-ELK3060MS-96, EBT-ELK3060MS-48, EBT-ELK3060MS-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Human
Alternative Synonym: NMMHCB, Cellular myosin heavy chain, type B, Myosin heavy chain, non-muscle IIb
Konzentration: 10 ng/mL
Detektionsbereich: 0.16-10 ng/mL
Sensitivitaet: 0.054 ng/mL
UniProt: P35580
Proben: Serum, plasma and other biological fluids.
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Metabolic pathway,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MYH10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MYH10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MYH10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human MYH10 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK3060MS