Human GREM1(Gremlin 1) ELISA Kit

Artikelnummer: EBT-ELK3100
Artikelname: Human GREM1(Gremlin 1) ELISA Kit
Artikelnummer: EBT-ELK3100
Hersteller Artikelnummer: ELK3100
Alternativnummer: EBT-ELK3100-96, EBT-ELK3100-48, EBT-ELK3100-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Human
Alternative Synonym: PIG2, CKTSF1B1, DAND2, IHG-2, DAND2, DRM, Increased in high glucose protein 2, Cell proliferation-inducing gene 2 protein, Cysteine knot superfamily 1, BMP antagonist 1
Konzentration: 8000 pg/mL
Detektionsbereich: 125-8000 pg/mL
Sensitivitaet: 55 pg/mL
UniProt: O60565
Proben: serum, plasma, tissue homogenates, urine and other biological fluids
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Signal transduction,Tumor immunity,Developmental science,Bone metabolism,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GREM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GREM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GREM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human GREM1 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK3100