Human ALDH1A1(Aldehyde Dehydrogenase 1 Family, Member A1) ELISA Kit

Artikelnummer: EBT-ELK3311
Artikelname: Human ALDH1A1(Aldehyde Dehydrogenase 1 Family, Member A1) ELISA Kit
Artikelnummer: EBT-ELK3311
Hersteller Artikelnummer: ELK3311
Alternativnummer: EBT-ELK3311-96, EBT-ELK3311-48, EBT-ELK3311-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Human
Alternative Synonym: ALDH11, ALDH1, ALDC, PUMB1, RALDH1, Retinaldehyde Dehydrogenase 1, Aldehyde dehydrogenase, cytosolic, Retinal dehydrogenase 1
Konzentration: 100 ng/mL
Detektionsbereich: 1.57-100 ng/mL
Sensitivitaet: 0.59 ng/mL
UniProt: P00352
Proben: Serum, plasma, tissue homogenates and other biological fluids
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Metabolic pathway,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ALDH1A1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ALDH1A1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ALDH1A1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human ALDH1A1 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK3311