Human CYR61(Cysteine Rich Protein, Angiogenic Inducer 61) ELISA Kit

Artikelnummer: EBT-ELK3410
Artikelname: Human CYR61(Cysteine Rich Protein, Angiogenic Inducer 61) ELISA Kit
Artikelnummer: EBT-ELK3410
Hersteller Artikelnummer: ELK3410
Alternativnummer: EBT-ELK3410-96, EBT-ELK3410-48, EBT-ELK3410-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Human
Alternative Synonym: CCN1, GIG1, IGFBP10, CCN family member 1, Insulin-like growth factor-binding protein 10
Konzentration: 4000 pg/mL
Detektionsbereich: 62.5-4000 pg/mL
Sensitivitaet: 25.9 pg/mL
UniProt: O00622
Proben: serum, plasma, tissue homogenates, cell lysates and other biological fluids
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Tumor immunity,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CYR61. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CYR61. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CYR61, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human CYR61 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK3410