Human ADAM22(A Disintegrin And Metalloprotease 22) ELISA Kit

Artikelnummer: EBT-ELK3619
Artikelname: Human ADAM22(A Disintegrin And Metalloprotease 22) ELISA Kit
Artikelnummer: EBT-ELK3619
Hersteller Artikelnummer: ELK3619
Alternativnummer: EBT-ELK3619-96, EBT-ELK3619-48, EBT-ELK3619-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Human
Alternative Synonym: MDC2, Disintegrin And Metalloproteinase Domain-Containing Protein 22, Metalloproteinase-Like,Disintegrin-Like,And Cysteine-Rich Protein 2
Konzentration: 10 ng/mL
Detektionsbereich: 0.16-10 ng/mL
Sensitivitaet: 0.061 ng/mL
UniProt: Q9P0K1
Proben: Serum, plasma, tissue homogenates and other biological fluids
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Enzyme & Kinase,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ADAM22. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ADAM22. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ADAM22, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human ADAM22 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK3619