Human SMN2(Survival Of Motor Neuron 2, Centromeric) Microsample ELISA Kit

Artikelnummer: EBT-ELK3655MS
Artikelname: Human SMN2(Survival Of Motor Neuron 2, Centromeric) Microsample ELISA Kit
Artikelnummer: EBT-ELK3655MS
Hersteller Artikelnummer: ELK3655MS
Alternativnummer: EBT-ELK3655MS-96, EBT-ELK3655MS-48, EBT-ELK3655MS-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Human
Alternative Synonym: SMNC, SMNT, SMA2, BCD541, SMA3, SMA4, SMA1, SMA
Konzentration: 10 ng/mL
Detektionsbereich: 0.16-10 ng/mL
Sensitivitaet: 0.061 ng/mL
Proben: Tissue homogenates and other biological fluids.
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Neuro science,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SMN2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SMN2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SMN2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human SMN2 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK3655MS