Human EAAT1(Excitatory Amino Acid Transporter 1) ELISA Kit

Artikelnummer: EBT-ELK3929
Artikelname: Human EAAT1(Excitatory Amino Acid Transporter 1) ELISA Kit
Artikelnummer: EBT-ELK3929
Hersteller Artikelnummer: ELK3929
Alternativnummer: EBT-ELK3929-96, EBT-ELK3929-48, EBT-ELK3929-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Human
Alternative Synonym: SLC1A3, EA6, GLAST1, Sodium-dependent glutamate/aspartate transporter 1, Solute Carrier 1 Member 3,Glial High Affinity Glutamate Transporter, GLutamate Aspartate Transporter
Konzentration: 20 ng/mL
Detektionsbereich: 0.32-20 ng/mL
Sensitivitaet: 0.121 ng/mL
UniProt: P43003
Proben: Tissue homogenates and other biological fluids.
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Neuro science,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EAAT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EAAT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EAAT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human EAAT1 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK3929