Human cTnT/TNNT2(Troponin T Type 2, Cardiac) ELISA Kit

Artikelnummer: EBT-ELK3961
Artikelname: Human cTnT/TNNT2(Troponin T Type 2, Cardiac) ELISA Kit
Artikelnummer: EBT-ELK3961
Hersteller Artikelnummer: ELK3961
Alternativnummer: EBT-ELK3961-96, EBT-ELK3961-48, EBT-ELK3961-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Human
Alternative Synonym: CMH2, cTnT, TnTc, Cardiomyopathy,Hypertrophic 2, Cardiac muscle troponin T, TNNT2, Troponin T Type 2, Cardiac
Konzentration: 1000 pg/mL
Detektionsbereich: 15.63-1000 pg/mL
Sensitivitaet: 6.7 pg/mL
UniProt: P45379
Proben: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Cardiovascular biology,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human cTnT/TNNT2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human cTnT/TNNT2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human cTnT/TNNT2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human cTnT/TNNT2 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK3961