Rat S100A10(S100 Calcium Binding Protein A10) ELISA Kit

Artikelnummer: EBT-ELK4262
Artikelname: Rat S100A10(S100 Calcium Binding Protein A10) ELISA Kit
Artikelnummer: EBT-ELK4262
Hersteller Artikelnummer: ELK4262
Alternativnummer: EBT-ELK4262-96, EBT-ELK4262-48, EBT-ELK4262-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Rat
Alternative Synonym: ANX2L, ANX2LG, CAL1L, CLP11, GP11, P11, P10, Calpactin-1 light chain, Cellular ligand of annexin II, Annexin II Ligand,Calpactin I,Light Polypeptide
Konzentration: 20 ng/mL
Detektionsbereich: 0.32-20 ng/mL
Sensitivitaet: 0.111 ng/mL
UniProt: P05943
Proben: Tissue homogenates, cell lysates and other biological fluids.
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Signal transduction,Tumor immunity,Neuro science,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat S100A10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat S100A10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat S100A10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat S100A10 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK4262