Mouse APP(Amyloid Precursor Protein) ELISA Kit

Artikelnummer: EBT-ELK4272
Artikelname: Mouse APP(Amyloid Precursor Protein) ELISA Kit
Artikelnummer: EBT-ELK4272
Hersteller Artikelnummer: ELK4272
Alternativnummer: EBT-ELK4272-96, EBT-ELK4272-48, EBT-ELK4272-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Mouse
Alternative Synonym: AAA, ABeta, ABPP, AD1, APPI, CVAP, PN2, CTFgamma, Peptidase Nexin-II Alzheimer Disease, Peptidase Nexin-II, Amyloid Beta(A4)Precursor Protein
Konzentration: 20 ng/mL
Detektionsbereich: 0.32-20 ng/mL
Sensitivitaet: 0.117 ng/mL
UniProt: P12023
Proben: Serum, plasma, tissue homogenates and other biological fluids
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Neuro science,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse APP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse APP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse APP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Mouse APP in the samples is then determined by comparing the OD of the samples to the standard curve
ELK4272