Mouse GCLM(Glutamate Cysteine Ligase, Modifier Subunit) ELISA Kit

Artikelnummer: EBT-ELK4338
Artikelname: Mouse GCLM(Glutamate Cysteine Ligase, Modifier Subunit) ELISA Kit
Artikelnummer: EBT-ELK4338
Hersteller Artikelnummer: ELK4338
Alternativnummer: EBT-ELK4338-96, EBT-ELK4338-48, EBT-ELK4338-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Mouse
Alternative Synonym: GLCLR, GECS, GCL, Gamma Glutamylcysteine Synthetase, Glutamate Cysteine Ligase Regulatory, Gamma-ECS regulatory subunit, Glutamate cysteine ligase modifier subunit
Konzentration: 10 ng/mL
Detektionsbereich: 0.16-10 ng/mL
Sensitivitaet: 0.071 ng/mL
UniProt: O09172
Proben: Serum, plasma, tissue homogenates and other biological fluids.
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Enzyme & Kinase,Hematology,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GCLM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GCLM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GCLM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Mouse GCLM in the samples is then determined by comparing the OD of the samples to the standard curve
ELK4338