Human AIM1(Absent In Melanoma 1) ELISA Kit

Artikelnummer: EBT-ELK4371
Artikelname: Human AIM1(Absent In Melanoma 1) ELISA Kit
Artikelnummer: EBT-ELK4371
Hersteller Artikelnummer: ELK4371
Alternativnummer: EBT-ELK4371-96, EBT-ELK4371-48, EBT-ELK4371-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Human
Alternative Synonym: ST4, CRYBG1, Suppression Of Tumorigenicity 4, Beta-Gamma Crystallin Domain Containing 1
Konzentration: 20 ng/mL
Detektionsbereich: 0.32-20 ng/mL
Sensitivitaet: 0.131 ng/mL
UniProt: Q9Y4K1
Proben: Tissue homogenates and other biological fluids.
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Tumor immunity,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human AIM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AIM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human AIM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human AIM1 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK4371