Human TAP1(Antigen Peptide Transporter 1) ELISA Kit

Artikelnummer: EBT-ELK4394
Artikelname: Human TAP1(Antigen Peptide Transporter 1) ELISA Kit
Artikelnummer: EBT-ELK4394
Hersteller Artikelnummer: ELK4394
Alternativnummer: EBT-ELK4394-96, EBT-ELK4394-48, EBT-ELK4394-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Human
Alternative Synonym: APT1, ABC17, ABCB2, PSF1, RING4, Y3, TAP1N, Peptide Supply Factor 1, Atp-Binding Cassette,Subfamily B,Member 2, Peptide transporter involved in antigen processing 1
Konzentration: 20 ng/mL
Detektionsbereich: 0.32-20 ng/mL
Sensitivitaet: 0.141 ng/mL
UniProt: Q03518
Proben: Tissue homogenates, cell lysates and other biological fluids.
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Immune molecule,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TAP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TAP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TAP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human TAP1 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK4394