Human ITGaM(Integrin Alpha M) ELISA Kit

Artikelnummer: EBT-ELK4618
Artikelname: Human ITGaM(Integrin Alpha M) ELISA Kit
Artikelnummer: EBT-ELK4618
Hersteller Artikelnummer: ELK4618
Alternativnummer: EBT-ELK4618-96, EBT-ELK4618-48, EBT-ELK4618-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Human
Alternative Synonym: CD11b, ITG-AM, CR3A, MAC-1, MAC1A, MO1A, Complement Component 3 Receptor 3 Subunit, Cell surface glycoprotein MAC-1 subunit alpha, Neutrophil adherence receptor
Konzentration: 2000 pg/mL
Detektionsbereich: 31.25-2000 pg/mL
Sensitivitaet: 13.5 pg/mL
UniProt: P11215
Proben: Serum, plasma, tissue homogenates and other biological fluids
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: CD & Adhesion molecule,Tumor immunity,Infection immunity,Immune molecule,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ITGaM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ITGaM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ITGaM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human ITGaM in the samples is then determined by comparing the OD of the samples to the standard curve
ELK4618