Human ABCC2(ATP Binding Cassette Transporter C2) ELISA Kit

Artikelnummer: EBT-ELK4725
Artikelname: Human ABCC2(ATP Binding Cassette Transporter C2) ELISA Kit
Artikelnummer: EBT-ELK4725
Hersteller Artikelnummer: ELK4725
Alternativnummer: EBT-ELK4725-96, EBT-ELK4725-48, EBT-ELK4725-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Human
Alternative Synonym: CMOAT1, ABC30, CMOAT, DJS, MRP2, cMRP, Canalicular Multispecific Organic Anion transporter 1, Canalicular multidrug resistance protein, Multidrug resistance-associated 2
Konzentration: 10 ng/mL
Detektionsbereich: 0.16-10 ng/mL
Sensitivitaet: 0.064 ng/mL
UniProt: Q92887
Proben: Tissue homogenates, cell lysates and other biological fluids.
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Signal transduction,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ABCC2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ABCC2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ABCC2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human ABCC2 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK4725