Human ABCC4(ATP Binding Cassette Transporter C4) ELISA Kit

Artikelnummer: EBT-ELK4732
Artikelname: Human ABCC4(ATP Binding Cassette Transporter C4) ELISA Kit
Artikelnummer: EBT-ELK4732
Hersteller Artikelnummer: ELK4732
Alternativnummer: EBT-ELK4732-96, EBT-ELK4732-48, EBT-ELK4732-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Human
Alternative Synonym: ABC-C4, MRP4, MOATB, Canalicular Multispecific Organic Anion Transporter B, Multidrug Resistance-Associated Protein 4, Multi-specific organic anion transporter B
Konzentration: 20 ng/mL
Detektionsbereich: 0.32-20 ng/mL
Sensitivitaet: 0.132 ng/mL
UniProt: O15439
Proben: tissue homogenates, cell lysates and other biological fluids
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Signal transduction,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ABCC4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ABCC4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ABCC4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human ABCC4 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK4732