Human PIK3C3(Phosphoinositide-3-Kinase Class 3) ELISA Kit

Produktübersicht

• Artikelname: Human PIK3C3(Phosphoinositide-3-Kinase Class 3) ELISA Kit
• Artikelnummer: EBT-ELK5138
• Hersteller Artikelnummer: ELK5138
• Alternativnummer: EBT-ELK5138-96, EBT-ELK5138-48, EBT-ELK5138-96X5
• Hersteller: ELK Biotechnology
• Kategorie: Kits/Assays
• Spezies Reaktivität: Human
• Alternative Synonym: Vps34, Phosphatidylinositol 3-kinase p100 subunit, Phosphatidylinositol 3-kinase catalytic subunit type 3

Produkteigenschaften

• Konzentration: 100 ng/mL
• Detektionsbereich: 1.57-100 ng/mL
• Sensitivitaet: 0.59 ng/mL
• UniProt: Q8NEB9
• Proben: Tissue homogenates, cell lysates and other biological fluids

Anwendungsbeschreibung

• Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Enzyme & Kinase,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PIK3C3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PIK3C3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PIK3C3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human PIK3C3 in the samples is then determined by comparing the OD of the samples to the standard curve

Bilder

ELK5138