Human XPD(Xeroderma Pigmentosum, Complementation Group D) ELISA Kit

Artikelnummer: EBT-ELK5232
Artikelname: Human XPD(Xeroderma Pigmentosum, Complementation Group D) ELISA Kit
Artikelnummer: EBT-ELK5232
Hersteller Artikelnummer: ELK5232
Alternativnummer: EBT-ELK5232-96, EBT-ELK5232-48, EBT-ELK5232-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Human
Alternative Synonym: CXPD, BTF2 p80, EM9, TTD, ERCC2, TFIIH basal transcription factor complex 80 kDa, De Sanctis-Cacchione, Excision Repair Cross-Complementing Rodent Repair Deficiency 2
Konzentration: 10 ng/mL
Detektionsbereich: 0.16-10 ng/mL
Sensitivitaet: 0.059 ng/mL
UniProt: P18074
Proben: Tissue homogenates, cell lysates and other biological fluids.
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Signal transduction,Tumor immunity,Dermatology,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human XPD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human XPD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human XPD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human XPD in the samples is then determined by comparing the OD of the samples to the standard curve
ELK5232