Rat GnRH(Gonadotropin Releasing Hormone) ELISA Kit

Artikelnummer: EBT-ELK5453
Artikelname: Rat GnRH(Gonadotropin Releasing Hormone) ELISA Kit
Artikelnummer: EBT-ELK5453
Hersteller Artikelnummer: ELK5453
Alternativnummer: EBT-ELK5453-96, EBT-ELK5453-48, EBT-ELK5453-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Rat
Alternative Synonym: GNRH1, GRH, LNRH, LHRH, LH-RH I, Luliberin, Luteinizing-Hormone Releasing Hormone, Progonadoliberin-1, Gonadoliberin I, Gonadorelin, GnRH-associated peptide 1
Konzentration: 1000 pg/mL
Detektionsbereich: 15.63-1000 pg/mL
Sensitivitaet: 5.13 pg/mL
UniProt: P07490
Proben: serum, plasma, cell lysates, cell culture supernates and other biological fluids
Anwendungsbeschreibung: Assay Type: Competitive Inhibition. Assay length: 2h. Research Area: Endocrinology,Reproductive science,Neuro science,Genetic science,Hormone metabolism,. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat GnRH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GnRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat GnRH in the samples is then determined by comparing the OD of the samples to the standard curve
ELK5453