Human PIIICP(Procollagen III C-Terminal Propeptide) ELISA Kit

Artikelnummer: EBT-ELK5493
Artikelname: Human PIIICP(Procollagen III C-Terminal Propeptide) ELISA Kit
Artikelnummer: EBT-ELK5493
Hersteller Artikelnummer: ELK5493
Alternativnummer: EBT-ELK5493-96, EBT-ELK5493-48, EBT-ELK5493-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Human
Alternative Synonym: P3CP, C-Propeptide Of Type III Procollagen, Procollagen III Carboxy Terminal Propeptide
Konzentration: 200 ng/mL
Detektionsbereich: 3.13-200 ng/mL
Sensitivitaet: 0.96 ng/mL
Proben: serum, plasma, cell lysates, cell culture supernates and other biological fluids
Anwendungsbeschreibung: Assay Type: Competitive Inhibition. Assay length: 2h. Research Area: Metabolic pathway,Bone metabolism,. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human PIIICP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PIIICP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human PIIICP in the samples is then determined by comparing the OD of the samples to the standard curve
ELK5493