Human PIIICP(Procollagen III C-Terminal Propeptide) ELISA Kit
Artikelnummer:
EBT-ELK5493
Hersteller Artikelnummer:
ELK5493
Alternativnummer:
EBT-ELK5493-96, EBT-ELK5493-48, EBT-ELK5493-96X5
Hersteller:
ELK Biotechnology
Kategorie:
Kits/Assays
Spezies Reaktivität:
Human
Alternative Synonym:
P3CP, C-Propeptide Of Type III Procollagen, Procollagen III Carboxy Terminal Propeptide
Konzentration:
200 ng/mL
Detektionsbereich:
3.13-200 ng/mL
Sensitivitaet:
0.96 ng/mL
Proben:
serum, plasma, cell lysates, cell culture supernates and other biological fluids
Anwendungsbeschreibung:
Assay Type: Competitive Inhibition. Assay length: 2h. Research Area: Metabolic pathway,Bone metabolism,. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human PIIICP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PIIICP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human PIIICP in the samples is then determined by comparing the OD of the samples to the standard curve
ELK5493
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