Rat IL-2sRa/CD25(Soluble Interleukin-2 Receptor alpha chain) Microsample ELISA Kit

Produktübersicht

• Artikelname: Rat IL-2sRa/CD25(Soluble Interleukin-2 Receptor alpha chain) Microsample ELISA Kit
• Artikelnummer: EBT-ELK5694MS
• Hersteller Artikelnummer: ELK5694MS
• Alternativnummer: EBT-ELK5694MS-96, EBT-ELK5694MS-48, EBT-ELK5694MS-96X5
• Hersteller: ELK Biotechnology
• Kategorie: Kits/Assays
• Spezies Reaktivität: Rat
• Alternative Synonym: IL-2RA,CD25, IL2R-a, IL2-RA, IDDM10, IL2R, TCGFR, p55, TAC antigen, IL2Ra, Interleukin 2 Receptor Alpha

Produkteigenschaften

• Konzentration: 10 ng/mL
• Detektionsbereich: 0.16-10 ng/mL
• Sensitivitaet: 0.06 ng/mL
• Proben: serum, plasma, tissue homogenates, cell lysates and other biological fluids

Anwendungsbeschreibung

• Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Signal transduction,CD & Adhesion molecule,Tumor immunity,Infection immunity,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IL-2sRa/CD25. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IL-2sRa/CD25. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IL-2sRa/CD25, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat IL-2sRa/CD25 in the samples is then determined by comparing the OD of the samples to the standard curve

Bilder

ELK5694MS