Rat ACE2(Angiotensin I Converting Enzyme 2) ELISA Kit

Artikelnummer: EBT-ELK5697
Artikelname: Rat ACE2(Angiotensin I Converting Enzyme 2) ELISA Kit
Artikelnummer: EBT-ELK5697
Hersteller Artikelnummer: ELK5697
Alternativnummer: EBT-ELK5697-96, EBT-ELK5697-48, EBT-ELK5697-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Rat
Alternative Synonym: ACEH, ACEII, ACE-II, Peptidyl-Dipeptidase A, ACE-related carboxypeptidase, Angiotensin-converting enzyme homolog, Metalloprotease MPROT15
Konzentration: 50 ng/mL
Detektionsbereich: 0.79-50 ng/mL
Sensitivitaet: 0.32 ng/mL
UniProt: Q5EGZ1
Proben: Serum, plasma, tissue homogenates and other biological fluids
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Enzyme & Kinase,Metabolic pathway,Endocrinology,Cardiovascular biology,Neuro science,Hepatology,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ACE2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ACE2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ACE2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat ACE2 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK5697