Pig Cys-C(Cystatin C) ELISA Kit

Artikelnummer: EBT-ELK5704
Artikelname: Pig Cys-C(Cystatin C) ELISA Kit
Artikelnummer: EBT-ELK5704
Hersteller Artikelnummer: ELK5704
Alternativnummer: EBT-ELK5704-96, EBT-ELK5704-48, EBT-ELK5704-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Porcine
Alternative Synonym: CST3, Cystatin 3, ARMD11, Post-Gamma-Globulin, Gamma Trace, Post-Gamma-Globulin, Neuroendocrine Basic Polypeptide, Amyloid Angiopathy And Cerebral Hemorrhage
Konzentration: 800 ng/mL
Detektionsbereich: 12.5-800 ng/mL
Sensitivitaet: 5.8 ng/mL
Proben: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Kidney biomarker,Urology,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig Cys-C. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig Cys-C. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig Cys-C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Pig Cys-C in the samples is then determined by comparing the OD of the samples to the standard curve
ELK5704