Pig SELP(Selectin, Platelet) ELISA Kit

Artikelnummer: EBT-ELK5743
Artikelname: Pig SELP(Selectin, Platelet) ELISA Kit
Artikelnummer: EBT-ELK5743
Hersteller Artikelnummer: ELK5743
Alternativnummer: EBT-ELK5743-96, EBT-ELK5743-48, EBT-ELK5743-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Porcine
Alternative Synonym: CD62P, P-Selectin, PSEL, GMP140, LECAM3, GRMP, GMRP, PADGEM, Leukocyte-endothelial cell adhesion molecule 3, Platelet activation dependent granule-external membrane
Konzentration: 400 ng/mL
Detektionsbereich: 6.25-400 ng/mL
Sensitivitaet: 2.62 ng/mL
Proben: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: CD & Adhesion molecule,Tumor immunity,Infection immunity,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig SELP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig SELP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig SELP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Pig SELP in the samples is then determined by comparing the OD of the samples to the standard curve
ELK5743