Rat GDF15(Growth Differentiation Factor 15) Microsample ELISA Kit

Artikelnummer: EBT-ELK5775MS
Artikelname: Rat GDF15(Growth Differentiation Factor 15) Microsample ELISA Kit
Artikelnummer: EBT-ELK5775MS
Hersteller Artikelnummer: ELK5775MS
Alternativnummer: EBT-ELK5775MS-96, EBT-ELK5775MS-48, EBT-ELK5775MS-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Rat
Alternative Synonym: PDF, MIC1, NAG-1, PLAB, PTGFB, Macrophage inhibitory cytokine 1, NSAID-activated gene 1 protein, Placental bone morphogenetic protein, Prostate differentiation factor
Konzentration: 1000 pg/mL
Detektionsbereich: 15.63-1000 pg/mL
Sensitivitaet: 6 pg/mL
UniProt: Q9Z0J6
Proben: Serum, plasma, tissue homogenates and other biological fluids.
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Cytokine,Apoptosis,Tumor immunity,Infection immunity,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat GDF15. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GDF15. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat GDF15, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat GDF15 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK5775MS