Rat SPC(Surfactant Associated Protein C) ELISA Kit

Artikelnummer: EBT-ELK5794
Artikelname: Rat SPC(Surfactant Associated Protein C) ELISA Kit
Artikelnummer: EBT-ELK5794
Hersteller Artikelnummer: ELK5794
Alternativnummer: EBT-ELK5794-96, EBT-ELK5794-48, EBT-ELK5794-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Rat
Alternative Synonym: SFTPC, PSP-C, SFTP2, SP-C, SP5, Pulmonary Surfactant Protein C, Pulmonary surfactant-associated proteolipid SPL(Val)
Konzentration: 20 ng/mL
Detektionsbereich: 0.32-20 ng/mL
Sensitivitaet: 0.117 ng/mL
UniProt: P11685
Proben: Tissue homogenates, cell lysates, lung lavage fluid and other biological fluids.
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Tumor immunity,Infection immunity,Pulmonology,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SPC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SPC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SPC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat SPC in the samples is then determined by comparing the OD of the samples to the standard curve
ELK5794