Cattle ADP(Adiponectin) ELISA Kit

Artikelnummer: EBT-ELK5893
Artikelname: Cattle ADP(Adiponectin) ELISA Kit
Artikelnummer: EBT-ELK5893
Hersteller Artikelnummer: ELK5893
Alternativnummer: EBT-ELK5893-96, EBT-ELK5893-48, EBT-ELK5893-96X5
Hersteller: ELK Biotechnology
Kategorie: Kits/Assays
Spezies Reaktivität: Bovine
Alternative Synonym: GBP28, ApM1, AdipoQ, Acrp30, ACDC, APM1, C1Q And Collagen Domain Containing, Adipocyte Complement-Related Protein Of 30 KDa, Adipose Most Abundant Gene Transcript 1
Konzentration: 50 ng/mL
Detektionsbereich: 0.79-50 ng/mL
Sensitivitaet: 0.34 ng/mL
UniProt: Q3Y5Z3
Proben: Serum, plasma and other biological fluids
Anwendungsbeschreibung: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Metabolic pathway,Infection immunity,Cardiovascular biology,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle ADP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle ADP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle ADP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Cattle ADP in the samples is then determined by comparing the OD of the samples to the standard curve
ELK5893